Construction and functional analysis of the core microbial community for omethoate degradation

Modern molecular biotechnology can analyze microbial communities further compared with traditional microbial research． Bioinformatics analysis of microbial community samples was conducted for domestication of garden soil with Omega, to explore the response law of soil microbial community structure to Omega, and to analyze the functions of core microbial communities． During domestication, the community was found to have completed a directional shift towards the degradation and adaptation of omethoate． As the degradation efficiency gradually improved, the degradation rate of omethoate reached over 90% at 24 days． From 18th to 24th days, the community structure reached a stable state at the phylum level, with the dominant phylum being Bacteroidetes, Proteobacteria, Acidobacteria and Gemmatimonadetes, the dominant communities at the genus level were Flavobacterium, Stenotrophobacter, Pseudomonas, Sphingomonas, Nitrospira． By modularizing the co-occurrence network and dividing the top three contributing modules into core functional bacterial communities, it was inferred that the core bacterial genera played a dominant role in community changes． Ferruginibacter was found to have contributed the greatest, Flavobacterium had the strongest inter group connectivity． Combined with PICRUSt2, functional predictions were made for the dominant communities and core functional bacterial communities at different stages of domestication． It was found that at the primary functional level, metabolic function was the most important, but at the secondary functional level, significant differences were present; these were mainly reflected in the high growth and passage levels and high secondary metabolite biosynthesis levels of core functional bacterial communities, to achieve complementary metabolic functions．